<Tr> <Th> Phenotype </Th> <Td> White - spotted (AA) </Td> <Td> Intermediate (Aa) </Td> <Td> Little spotting (aa) </Td> <Th> Total </Th> </Tr> <Tr> <Th> Number </Th> <Td> 1469 </Td> <Td> 138 </Td> <Td> 5 </Td> <Td> 1612 </Td> </Tr> <P> From this, allele frequencies can be calculated: </P> <Dl> <Dd> p = 2 × o b s (AA) + o b s (Aa) 2 × (o b s (AA) + o b s (Aa) + o b s (aa)) = 1469 × 2 + 138 2 × (1469 + 138 + 5) = 3076 3224 = 0.954 (\ displaystyle (\ begin (aligned) p& = (2 \ times \ mathrm (obs) ((\ text (AA))) + \ mathrm (obs) ((\ text (Aa))) \ over 2 \ times (\ mathrm (obs) ((\ text (AA))) + \ mathrm (obs) ((\ text (Aa))) + \ mathrm (obs) ((\ text (aa))))) \ \ \ \ & = (1469 \ times 2 + 138 \ over 2 \ times (1469 + 138 + 5)) \ \ \ \ & = (3076 \ over 3224) \ \ \ \ & = 0.954 \ end (aligned))) </Dd> </Dl>

What are the 5 assumptions of the hardy weinberg principle