<P> Termination of DNA replication in E. coli is completed through the use of termination sequences and the Tus protein . These sequences allow the two replication forks to pass through in only one direction, but not the other . </P> <P> DNA replication initially produces two catenated or linked circular DNA duplexes, each comprising one parental strand and one newly synthesised strand (by nature of semiconservative replication). This catenation can be visualised as two interlinked rings which cannot be separated . Topoisomerase 2 in E. coli unlinks or decatenates the two circular DNA duplexes . </P>

List and describe the steps in prokaryotic dna replication