<P> Neville's daily routine includes experimentation on infected rats to find a cure to the virus and venturing through Manhattan to search for food and supplies . Also awaiting for potential human survivors each day for a response to his continuous recorded radio broadcasts, which instruct any uninfected survivors to meet him at midday at the South Street Seaport . </P> <P> Flashbacks reveal that his wife (Salli Richardson) and daughter (Willow Smith) died in a helicopter accident during the chaotic evacuation of Manhattan, prior to the military - enforced quarantine of the island in 2009, in which Neville stays behind on the island as military personnel . Neville's loneliness is mitigated by the companionship of his German Shepherd Samantha aka Sam (given to him by his daughter Marley as a puppy to protect him before she died in the helicopter crash), interaction with mannequins he has set up as patrons at a video store, and recordings of old television broadcasts . At night, he barricades himself and Sam inside his heavily fortified Washington Square Park home to hide from the Darkseekers . One day, while waiting for survivors, Sam follows a deer into a dark building . Neville cautiously goes in after her and finds the deer's corpse along with Sam, but the building is infested by a colony of Darkseekers . Both manage to escape unharmed and the attacking Darkseekers are killed by the sunlight . </P> <P> Neville finds a promising treatment derived from his own blood, so he sets a snare trap and captures a female Darkseeker from the colony building, while a male Darkseeker (termed "Alpha Male" in the script, and played by Dash Mihok) tries to go after them, but is blocked by the sunlight and returns to the shadows . Back in his laboratory in the basement of his house, Neville treats the female without success . The next day, he is ensnared in a trap similar to the one he used to capture the female; by the time he manages to escape, the sun is setting and he is attacked by infected dogs . Neville and Sam manage to eliminate them, but Sam is bitten in the fight . Neville injects her with a strand of his serum, but when she shows signs of infection, Neville is forced to strangle her to death . Heartbroken and driven over the edge of insanity by his dog's death, he ventures out and suicidally attacks a group of Darkseekers the next night . He kills a large number of Darkseekers but the rest overwhelm him and he is nearly killed, but is rescued by a pair of immune survivors, Anna (Alice Braga) and a young boy named Ethan (Charlie Tahan), who have traveled from Maryland after hearing one of his broadcasts . They take the injured Neville back to his home, where Anna explains that they survived the outbreak aboard a Red Cross evacuation ship from São Paulo and are making their way to a survivors' camp in Bethel, Vermont . Neville angrily argues that no such survivors' camp exists . </P> <P> Neville once again attempts to administer a potential cure to the infected woman in his laboratory, but the next night, a group of Darkseekers, who had followed Anna and Neville back the night before, attacks the house . Neville, Anna, and Ethan retreat into the basement laboratory, sealing themselves in with the female Darkseeker on which Neville has been experimenting . Discovering that the last treatment was successful, Neville tries to assess the situation as the alpha male deliberately rams himself against a glass door to break in . Neville draws a vial of blood from the woman he cured and gives it to Anna, before shutting her and Ethan inside a coal chute in the back of the lab . He then takes a grenade and kills the Darkseekers at the cost of his own life, saving the cure . The next day, before dusk, Anna and Ethan discover that her theory is right as they arrive at the survivors camp in Vermont . They are greeted by military officers and other human survivors before Anna is shown handing the cure to military personnel . </P>

When does sam die in i am legend
find me the text answering this question