<P> The ends generated by NdeI digest: </P> <P> NdeI is a specific Type II restriction enzyme that cuts open specific target sequences, unlike exonucleases . This enzyme is used in gene cloning to cut open reading frames in the plasmid of certain bacteria such as E. coli and insert a foreign gene, such as the gfpuv gene that codes for bio fluorescence of the jelly fish Aequorea victoria . </P> <P> NdeI is useful in generating heterologous DNA construct because it contains the start codon ATG . It therefore can be in used in some expression vectors, such as those in the pET series of vectors, for the ligation of the start of a gene when making an expression construct . Note however that NdeI generates only a two - base overhang and therefore has a lower melting temperature than other restriction enzymes that generate a four - base overhang . It has a lower ligation efficiency, since ligation is affected by the ability of the ends to anneal and a two - base overhang has a significantly lower melting temperature compared to a 4 - base overhang . Ligation of NdeI - generated ends is therefore best performed with higher ligase concentration with a longer ligation time, whether at room temperature, 14 - 16 C, or at 4 C . </P>

What nucleotide sequences are recognized by the restriction enzymes ndei