<P> Photosynthetic water splitting (or oxygen evolution) is one of the most important reactions on the planet, since it is the source of nearly all the atmosphere's oxygen . Moreover, artificial photosynthetic water - splitting may contribute to the effective use of sunlight as an alternative energy - source . </P> <P> The mechanism of water oxidation is still not fully elucidated, but we know many details about this process . The oxidation of water to molecular oxygen requires extraction of four electrons and four protons from two molecules of water . The experimental evidence that oxygen is released through cyclic reaction of oxygen evolving complex (OEC) within one PSII was provided by Pierre Joliot et al. They have shown that, if dark - adapted photosynthetic material (higher plants, algae, and cyanobacteria) is exposed to a series of single turnover flashes, oxygen evolution is detected with typical period - four damped oscillation with maxima on the third and the seventh flash and with minima on the first and the fifth flash (for review, see). Based on this experiment, Bessel Kok and co-workers introduced a cycle of five flash - induced transitions of the so - called S - states, describing the four redox states of OEC: When four oxidizing equivalents have been stored (at the S - state), OEC returns to its basic S - state . In the absence of light, the OEC will "relax" to the S state; the S state is often described as being "dark - stable". The S state is largely considered to consist of manganese ions with oxidation states of Mn, Mn, Mn, Mn . Finally, the intermediate S - states were proposed by Jablonsky and Lazar as a regulatory mechanism and link between S - states and tyrosine Z . </P> <P> In 2012, Renger expressed the idea of internal changes of water molecules into typical oxides in different S - states during water splitting . In 2017, Dolai showed almost complete concept of water splitting by the way of structural analysis of S - states and developed oxides from the water molecules . </P> <P> Dolai's Diagram of S - states (Dolai's Mechanism): Dolai's diagrams are pictures of different S - states in water - splitting during photosynthesis . These diagrams can express estimated figures of intermediate S - states as well as the development of typical oxides (monoxide (2H2O), hydroxide (OH. H O), peroxide (H O), super oxide (HO) and di - oxygen (O)); which are formed due to interchange of water molecules in S - states . The arrangement of hydrogen bonding is main criteria of the diagrams . The effect of photon (hv), emission of electron (e) and production of hydrogen ion (H+) from water molecules can be shown by the diagrams in a more specific way of experimental detection . The diagrams almost completely analyze the pathway of water - splitting in photosynthesis . </P>

Where does splitting of water occur in photosynthesis