<Tr> <Td> Substance in water + 3ml Benedict's solution, then boil for few minutes and allow to cool . </Td> <Td> Solution remains clear or is a little blue </Td> <Td> Reducing sugar is not present </Td> </Tr> <P> Benedict's quantitative reagent contains potassium thiocyanate and is used to determine how much reducing sugar is present . This solution forms a copper thiocyanate precipitate which is white and can be used in a titration . The titration should be repeated with 1% glucose solution instead of the sample for calibration . </P>

When using copper to test for a reducing sugar the color will change