<P> This was later standardised at 68 ° F and then 20 ° C . This temperature may be significantly different from the temperature of the natural environment of the water being tested . </P> <P> Although the Royal Commission on Sewage Disposal proposed 5 days as an adequate test period for rivers of the United Kingdom of Great Britain and Ireland, longer periods were investigated for North American rivers . Incubation periods of 1, 2, 5, 10 and 20 days were being used into the mid-20th century . Keeping dissolved oxygen available at their chosen temperature, investigators found up to 99 percent of total BOD was exerted within 20 days, 90 percent within 10 days, and approximately 68 percent within 5 days . Variable microbial population shifts to nitrifying bacteria limit test reproducibility for periods greater than 5 days . The 5 - day test protocol with acceptably reproducible results emphasizing carbonaceous BOD has been endorsed by the United States Environmental Protection Agency . This 5 - day BOD test result may be described as the amount of oxygen required for aquatic microorganisms to stabilize decomposable organic matter under aerobic conditions . Stabilization, in this context, may be perceived in general terms as the conversion of food to living aquatic fauna . Although these fauna will continue to exert biochemical oxygen demand as they die, that tends to occur within a more stable evolved ecosystem including higher trophic levels . </P> <P> Most pristine rivers will have a 5 - day carbonaceous BOD below 1 mg / L. Moderately polluted rivers may have a BOD value in the range of 2 to 8 mg / L. Rivers may be considered severely polluted when BOD values exceed 8 mg / L. Municipal sewage that is efficiently treated by a three - stage process would have a value of about 20 mg / L or less . Untreated sewage varies, but averages around 600 mg / L in Europe and as low as 200 mg / L in the U.S., or where there is severe groundwater or surface water Infiltration / Inflow . The generally lower values in the U.S. derive from the much greater water use per capita than in other parts of the world . </P> <P> There are two commonly recognized methods for the measurement of BOD . </P>

Bod of raw municipal sewage may be in the range of about