<Tr> <Td> </Td> <Td> Wikinews has related news: Healthy cloned monkeys born in Shanghai </Td> </Tr> <P> It is a technique for cloning in which the nucleus of a somatic cell is transferred to the cytoplasm of an enucleated egg . When this is done, the cytoplasmic factors affect the nucleus to become a zygote . The blastocyst stage is developed by the egg which helps to create embryonic stem cells from the inner cell mass of the blastocyst . The first animal that was developed by this technique was Dolly, the sheep, in 1996 . </P> <P> The process of somatic cell nuclear transplant involves two different cells . The first being a female gamete, known as the ovum (egg / oocyte). In human SCNT (Somatic Cell Nuclear Transfer) experiments, these eggs are obtained through consenting donors, utilizing ovarian stimulation . The second being a somatic cell, referring to the cells of the human body . Skin cells, fat cells, and liver cells are only a few examples . The nucleus of the donor egg cell is removed and discarded, leaving it' deprogrammed .' What is left is a somatic cell and an denucleated egg cell . These are then fused by inserting the somatic cell into the' empty' ovum . After being inserted into the egg, the somatic cell nucleus is reprogrammed by its host egg cell . The ovum, now containing the somatic cell's nucleus, is stimulated with a shock and will begin to divide . The egg is now viable and capable of producing an adult organism containing all the necessary genetic information from just one parent . Development will ensue normally and after many mitotic divisions, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with an identical genome to the original organism (i.e. a clone). Stem cells can then be obtained by the destruction of this clone embryo for use in therapeutic cloning or in the case of reproductive cloning the clone embryo is implanted into a host mother for further development and brought to term . </P> <P> Somatic cell nuclear transplantation has become a focus of study in stem cell research . The aim of carrying out this procedure is to obtain pluripotent cells from a cloned embryo . These cells genetically matched the donor organism from which they came . This gives them the ability to create patient specific pluripotent cells, which could then be used in therapies or disease research . </P>

What are the 4 steps of nuclear transfer to create a cloned organism
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