<Tr> <Td> </Td> <Td> This article needs additional citations for verification . Please help improve this article by adding citations to reliable sources . Unsourced material may be challenged and removed . (January 2009) (Learn how and when to remove this template message) </Td> </Tr> <P> Trypticase soy agar or tryptone soya agar (TSA) and Trypticase soy broth or tryptone soya broth (TSB) with agar are growth media for the culturing of bacteria . They are general - purpose, nonselective media providing enough nutrients to allow for a wide variety of microorganisms to grow . They are used for a wide range of applications, including culture storage, enumeration (counting), isolation of pure cultures, or simply general culture . </P> <P> TSA contains enzymatic digests of casein and soybean meal, which provide amino acids and other nitrogenous substances, making it a nutritious medium for a variety of organisms . Glucose is the energy source . Sodium chloride maintains the osmotic equilibrium, while dipotassium phosphate acts as buffer to maintain pH . Agar extracted from any number of organisms is used as a gelling agent . </P> <P> The medium may be supplemented with blood to facilitate the growth of more fastidious bacteria or antimicrobial agents to permit the selection of various microbial groups from pure microbiota . As with any media, minor changes may be made to suit specific circumstances . TSA is frequently the base medium of other agar plate types . For example, blood agar plates (BAP) are made by enriching TSA plates with defibrinated sheep blood, and chocolate agar is made through additional cooking of BAP . Nutrient agar is also similar to TSA . One liter of the agar contains: </P>

What are bacteria using for nutrients in t soy agar
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