<P> Utilization of wastewater as a potential substrate for biohydrogen production has been drawing considerable interest in recent years especially in the dark fermentation process . Industrial wastewater as a fermentative substrate for H production addresses most of the criteria required for substrate selection viz., availability, cost and biodegradability (Angenent, et al., 2004; Kapdan and Kargi, 2006). Chemical wastewater (Venkata Mohan, et al., 2007a, b), cattle wastewater (Tang, et al., 2008), dairy process wastewater (Venkata Mohan, et al. 2007c, Rai et al. 2012), starch hydrolysate wastewater (Chen, et al., 2008) and designed synthetic wastewater (Venkata Mohan, et al., 2007a, 2008b) have been reported to produce biohydrogen apart from wastewater treatment from dark fermentation processes using selectively enriched mixed cultures under acidophilic conditions . Various wastewaters viz., paper mill wastewater (Idania, et al., 2005), starch effluent (Zhang, et al., 2003), food processing wastewater (Shin et al., 2004, van Ginkel, et al., 2005), domestic wastewater (Shin, et al., 2004, 2008e), rice winery wastewater (Yu et al., 2002), distillery and molasses based wastewater (Ren, et al., 2007, Venkata Mohan, et al., 2008a), wheat straw wastes (Fan, et al., 2006) and palm oil mill wastewater (Vijayaraghavan and Ahmed, 2006) have been studied as fermentable substrates for H production along with wastewater treatment . Using wastewater as a fermentable substrate facilitates both wastewater treatment apart from H production . The efficiency of the dark fermentative H production process was found to depend on pre-treatment of the mixed consortia used as a biocatalyst, operating pH, and organic loading rate apart from wastewater characteristics (Venkata Mohan, et al., 2007d, 2008c, d, Vijaya Bhaskar, et al., 2008d). </P> <P> In spite of its advantages, the main challenge observed with fermentative H production processes is the relatively low energy conversion efficiency from the organic source . Typical H yields range from 1 to 2 mol of H / mol of glucose, which results in 80 - 90% of the initial COD remaining in the wastewater in the form of various volatile organic acids (VFAs) and solvents, such as acetic acid, propionic acid, butyric acid, and ethanol . Even under optimal conditions about 60 - 70% of the original organic matter remains in solution . Bioaugmentation with selectively enriched acidogenic consortia to enhance H production was also reported (Venkata Mohan, et al., 2007b). Generation and accumulation of soluble acid metabolites causes a sharp drop in the system pH and inhibits the H production process . Usage of unutilized carbon sources present in acidogenic process for additional biogas production sustains the practical applicability of the process . One way to utilize / recover the remaining organic matter in a usable form is to produce additional H by terminal integration of photo - fermentative processes of H production (Venkata Mohan, et al. 2008e, Rai et al. 2012) and methane by integrating acidogenic processes to terminal methanogenic processes . </P>

What do you understand by the term dark fermentation