<P> Translation initiation happens following recruitment of the ribosome, at the start codon (underlined) found within the Kozak consensus sequence ACCAUGG . Since the Kozak sequence itself is not involved in the recruitment of the ribosome, it is not considered a ribosome binding site . </P> <P> Eukaryotic ribosomes are known to bind to transcripts in a mechanism unlike the one involving the 5' cap, at a sequence called the internal ribosome entry site . This process is not dependent on the full set of translation initiation factors (although this depends on the specific IRES) and is commonly found in the translation of viral mRNA . </P> <P> The identification of RBSs is used to determine the site of translation initiation in an unannotated sequence . This is referred to as N - terminal prediction . This is especially useful when multiple start codons are situated around the potential start site of the protein coding sequence . </P> <P> Identification of RBSs is particularly difficult, because they tend to be highly degenerated . One approach to identifying RBS in E. coli is using neural networks . Another approach is using the Gibbs sampling method . </P>

Where does the ribosome bind to initiate translation