<P> One of the cell cycle checkpoints occurs during prometaphase and metaphase . Only after all chromosomes have become aligned at the metaphase plate, when every kinetochore is properly attached to a bundle of microtubules, does the cell enter anaphase . It is thought that unattached or improperly attached kinetochores generate a signal to prevent premature progression to anaphase, even if most of the kinetochores have been attached and most of the chromosomes have been aligned . Such a signal creates the mitotic spindle checkpoint . This would be accomplished by regulation of the anaphase - promoting complex, securin, and separase . </P> <P> The analysis of metaphase chromosomes is one of the main tools of classical cytogenetics and cancer studies . Chromosomes are condensed (thickened) and highly coiled in metaphase, which makes them most suitable for visual analysis . Metaphase chromosomes make the classical picture of chromosomes (karyotype). For classical cytogenetic analyses, cells are grown in short term culture and arrested in metaphase using mitotic inhibitor . Further they are used for slide preparation and banding (staining) of chromosomes to be visualised under microscope to study structure and number of chromosomes (karyotype). Staining of the slides, often with Giemsa (G banding) or Quinacrine, produces a pattern of in total up to several hundred bands . Normal metaphase spreads are used in methods like FISH and as a hybridization matrix for comparative genomic hybridization (CGH) experiments . </P> <P> Malignant cells from solid tumors or leukemia samples can also be used for cytogenetic analysis to generate metaphase preparations . Inspection of the stained metaphase chromosomes allows the determination of numerical and structural changes in the tumor cell genome, for example, losses of chromosomal segments or translocations, which may lead to chimeric oncogenes, such as bcr - abl in chronic myelogenous leukemia . </P>

How many chromosomes are at the metaphase 2 equator