<Li> The same cell processes the pre-mRNA strands by removing introns, and adding a poly - A tail and 5' Methyl - Guanine cap (this is known as post-transcriptional modification) </Li> <Li> This mixture of mature mRNA strands is extracted from the cell . The poly - A tail of the post-transcriptional mRNA can be taken advantage of with oligo (dT) beads in an affinity chromatography assay . </Li> <Li> A poly - T oligonucleotide primer is hybridized onto the poly - A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA (Reverse transcriptase requires this double - stranded segment as a primer to start its operation .) </Li> <Li> Reverse transcriptase is added, along with deoxynucleotide triphosphates (A, T, G, C). This synthesizes one complementary strand of DNA hybridized to the original mRNA strand . </Li>

Name the scientist who first initiated the sequencing of cdna molecules using mrna