<P> Free ribosomes can move about anywhere in the cytosol, but are excluded from the cell nucleus and other organelles . Proteins that are formed from free ribosomes are released into the cytosol and used within the cell . Since the cytosol contains high concentrations of glutathione and is, therefore, a reducing environment, proteins containing disulfide bonds, which are formed from oxidized cysteine residues, cannot be produced within it . </P> <P> When a ribosome begins to synthesize proteins that are needed in some organelles, the ribosome making this protein can become "membrane - bound". In eukaryotic cells this happens in a region of the endoplasmic reticulum (ER) called the "rough ER". The newly produced polypeptide chains are inserted directly into the ER by the ribosome undertaking vectorial synthesis and are then transported to their destinations, through the secretory pathway . Bound ribosomes usually produce proteins that are used within the plasma membrane or are expelled from the cell via exocytosis . </P> <P> In bacterial cells, ribosomes are synthesized in the cytoplasm through the transcription of multiple ribosome gene operons . In eukaryotes, the process takes place both in the cell cytoplasm and in the nucleolus, which is a region within the cell nucleus . The assembly process involves the coordinated function of over 200 proteins in the synthesis and processing of the four rRNAs, as well as assembly of those rRNAs with the ribosomal proteins . </P> <P> The ribosome may have first originated in an RNA world, appearing as a self - replicating complex that only later evolved the ability to synthesize proteins when amino acids began to appear . Studies suggest that ancient ribosomes constructed solely of rRNA could have developed the ability to synthesize peptide bonds . In addition, evidence strongly points to ancient ribosomes as self - replicating complexes, where the rRNA in the ribosomes had informational, structural, and catalytic purposes because it could have coded for tRNAs and proteins needed for ribosomal self - replication . Hypothetical cellular organisms with self - replicating RNA but without DNA are called ribocytes (or ribocells). </P>

Where is the rna of the ribosomes made