<P> The literal meaning of tissue culture refers to the culturing of tissue pieces, i.e. explant culture . </P> <P> Tissue culture is an important tool for the study of the biology of cells from multicellular organisms . It provides an in vitro model of the tissue in a well defined environment which can be easily manipulated and analysed . </P> <P> In animal tissue culture, cells may be grown as two - dimensional monolayers (conventional culture) or within fibrous scaffolds or gels to attain more naturalistic three - dimensional tissue - like structures (3D culture). Eric Simon, in a 1988 NIH SBIR grant report, showed that electrospinning could be used to produced nano - and submicron - scale polymeric fibrous scaffolds specifically intended for use as in vitro cell and tissue substrates . This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types would adhere to and proliferate upon polycarbonate fibers . It was noted that as opposed to the flattened morphology typically seen in 2D culture, cells grown on the electrospun fibers exhibited a more rounded 3 - dimensional morphology generally observed of tissues in vivo . </P> <P> Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium </P>

What do you mean by tissue culture in hindi