<P> In order to ligate these fragments together, the ligase progresses through three steps: </P> <Ol> <Li> Addition of an adenosine monophosphate (AMP) group to the enzyme, referred to as adenylylation, </Li> <Li> Adenosine monophosphate transfer to the DNA and </Li> <Li> Nick sealing, or phosphodiester bond formation . </Li> </Ol> <Li> Addition of an adenosine monophosphate (AMP) group to the enzyme, referred to as adenylylation, </Li> <Li> Adenosine monophosphate transfer to the DNA and </Li>

Responsible for the final binding of nicks in dna during replication