<Li> The eluite is the analyte, the eluted solute . </Li> <Li> An eluotropic series is a list of solvents ranked according to their eluting power . </Li> <Li> An immobilized phase is a stationary phase that is immobilized on the support particles, or on the inner wall of the column tubing . </Li> <Li> The mobile phase is the phase that moves in a definite direction . It may be a liquid (LC and Capillary Electrochromatography (CEC)), a gas (GC), or a supercritical fluid (supercritical - fluid chromatography, SFC). The mobile phase consists of the sample being separated / analyzed and the solvent that moves the sample through the column . In the case of HPLC the mobile phase consists of a non-polar solvent (s) such as hexane in normal phase or a polar solvent such as methanol in reverse phase chromatography and the sample being separated . The mobile phase moves through the chromatography column (the stationary phase) where the sample interacts with the stationary phase and is separated . </Li>

What is mobile and stationary phase in chromatography