<Ul> <Li> Where E is enzyme, S is substrate, and P is product </Li> </Ul> <Li> Where E is enzyme, S is substrate, and P is product </Li> <P> While the first (binding) and third (unbinding) steps are, in general, reversible, the middle step may be irreversible (as in the rennin and catalase reactions just mentioned) or reversible (e.g. many reactions in the glycolysis metabolic pathway). </P> <P> By increasing the substrate concentration, the rate of reaction will increase due to the likelihood that the number of enzyme - substrate complexes will increase; this occurs until the enzyme concentration becomes the limiting factor . </P>

What is the substrate in the reaction above