<P> Infections such as chlamydia, genital warts, syphilis, herpes simplex, and gonorrhea can also affect the vagina, but these diseases are found by doing other tests . </P> <P> It may also be used in a rape investigation to detect presence of semen . </P> <P> Vaginal wet mounts are not done during the menstrual period, because menstrual blood on the slide can confound the results . Vaginal irrigation, tampon use or sex (potentially disrupting the vaginal pH) should be avoided for 24 hours before the test . Vaginal medicines (such as a nonprescription vaginal yeast medicine) should not be used during the 2 to 3 days before the test . </P> <P> The sampling is done with the patient in lithotomy position . A speculum is used to facilitate use of a swab or spatula to sample fluid inside the vagina . The sampling procedure may cause some discomfort and minor bleeding, but otherwise there are no associated risks . The sample is then smeared upon a microscope slide and is observed by wet mount microscopy by placing the specimen on a glass slide and mixing with a salt solution . </P>

Wet mount positive for clue cells spores and hyphae