<Ul> <Li> 2 DNA Pol III enzymes, each comprising α, ε and θ subunits . (It has been proven that there is a third copy of Pol III at the replisome .) <Ul> <Li> the α subunit (encoded by the dnaE gene) has the polymerase activity . </Li> <Li> the ε subunit (dnaQ) has 3' → 5' exonuclease activity . </Li> <Li> the θ subunit (holE) stimulates the ε subunit's proofreading . </Li> </Ul> </Li> <Li> 2 β units (dnaN) which act as sliding DNA clamps, they keep the polymerase bound to the DNA . </Li> <Li> 2 τ units (dnaX) which acts to dimerize two of the core enzymes (α, ε, and θ subunits). </Li> <Li> 1 γ unit (also dnaX) which acts as a clamp loader for the lagging strand Okazaki fragments, helping the two β subunits to form a unit and bind to DNA . The γ unit is made up of 5 γ subunits which include 3 γ subunits, 1 δ subunit (holA), and 1 δ' subunit (holB). The δ is involved in copying of the lagging strand . </Li> <Li> Χ (holC) and Ψ (holD) which form a 1: 1 complex and bind to γ or τ . X can also mediate the switch from RNA primer to DNA . </Li> </Ul> <Li> 2 DNA Pol III enzymes, each comprising α, ε and θ subunits . (It has been proven that there is a third copy of Pol III at the replisome .) <Ul> <Li> the α subunit (encoded by the dnaE gene) has the polymerase activity . </Li> <Li> the ε subunit (dnaQ) has 3' → 5' exonuclease activity . </Li> <Li> the θ subunit (holE) stimulates the ε subunit's proofreading . </Li> </Ul> </Li> <Ul> <Li> the α subunit (encoded by the dnaE gene) has the polymerase activity . </Li> <Li> the ε subunit (dnaQ) has 3' → 5' exonuclease activity . </Li> <Li> the θ subunit (holE) stimulates the ε subunit's proofreading . </Li> </Ul> <Li> the α subunit (encoded by the dnaE gene) has the polymerase activity . </Li>

Dna polymerase iii is needed for adding nucleotides during mrna synthesis