<P> The cell envelope is composed of the plasma membrane and cell wall . As in other organisms, the bacterial cell wall provides structural integrity to the cell . In prokaryotes, the primary function of the cell wall is to protect the cell from internal turgor pressure caused by the much higher concentrations of proteins and other molecules inside the cell compared to its external environment . The bacterial cell wall differs from that of all other organisms by the presence of peptidoglycan which is located immediately outside of the cytoplasmic membrane . Peptidoglycan is made up of a polysaccharide backbone consisting of alternating N - Acetylmuramic acid (NAM) and N - acetylglucosamine (NAG) residues in equal amounts . Peptidoglycan is responsible for the rigidity of the bacterial cell wall and for the determination of cell shape . It is relatively porous and is not considered to be a permeability barrier for small substrates . While all bacterial cell walls (with a few exceptions e.g. extracellular parasites such as Mycoplasma) contain peptidoglycan, not all cell walls have the same overall structures . Since the cell wall is required for bacterial survival, but is absent in some eukaryotes, several antibiotics (notably the penicillins and cephalosporins) stop bacterial infections by interfering with cell wall synthesis, while having no effects on human cells which have no cell wall only a cell membrane . There are two main types of bacterial cell walls, those of gram - positive bacteria and those of gram - negative bacteria, which are differentiated by their Gram staining characteristics . For both these types of bacteria, particles of approximately 2 nm can pass through the peptidoglycan . If the bacterial cell wall is entirely removed, it is called a protoplast while if it's partially removed, it is called a spheroplast . β - Lactam antibiotics such as penicillin inhibit the formation of peptidoglycan cross-links in the bacterial cell wall . The enzyme lysozyme, found in human tears, also digests the cell wall of bacteria and is the body's main defense against eye infections . </P> <P> Gram - positive cell walls are thick and the peptidoglycan (also known as murein) layer constitutes almost 95% of the cell wall in some gram - positive bacteria and as little as 5 - 10% of the cell wall in gram - negative bacteria . The gram - positive bacteria take up the crystal violet dye and are stained purple . The cell wall of some gram - positive bacteria can be completely dissolved by lysozymes which attacks the bonds between GA and MA . In other gram - positive bacteria, such as Staphylococcus aureus, the walls are resistant to the action of lysozymes . They have O - acetyl groups on carbon - 6 of some MA residues . The matrix substances in the walls of gram - positive bacteria may be polysaccharides or teichoic acids . The latter are very widespread, but have been found only in gram - positive bacteria . There are two main types of teichoic acid: ribitol teichoic acids and glycerol teichoic acids . The latter one is more widespread . These acids are polymers of ribitol phosphate and glycerol phosphate, respectively, and only located on the surface of many gram - positive bacteria . However, the exact function of teichoic acid is debated and not fully understood . A major component of the gram - positive cell wall is lipoteichoic acid . One of its purposes is providing an antigenic function . The lipid element is to be found in the membrane where its adhesive properties assist in its anchoring to the membrane . </P> <P> Gram - negative cell walls are thin and unlike the gram - positive cell walls, they contain a thin peptidoglycan layer adjacent to the cytoplasmic membrane . Gram - negative bacteria are stained as pink colour . The chemical structure of the outer membrane's lipopolysaccharide is often unique to specific bacterial sub-species and is responsible for many of the antigenic properties of these strains . Lipopolysaccharides, also called endotoxins, are composed of polysaccharides and lipid A which are responsible for much of the toxicity of gram - negative bacteria . </P> <P> The plasma membrane or bacterial cytoplasmic membrane is composed of a phospholipid bilayer and thus has all of the general functions of a cell membrane such as acting as a permeability barrier for most molecules and serving as the location for the transport of molecules into the cell . In addition to these functions, prokaryotic membranes also function in energy conservation as the location about which a proton motive force is generated . Unlike eukaryotes, bacterial membranes (with some exceptions e.g. Mycoplasma and methanotrophs) generally do not contain sterols . However, many microbes do contain structurally related compounds called hopanoids which likely fulfill the same function . Unlike eukaryotes, bacteria can have a wide variety of fatty acids within their membranes . Along with typical saturated and unsaturated fatty acids, bacteria can contain fatty acids with additional methyl, hydroxy or even cyclic groups . The relative proportions of these fatty acids can be modulated by the bacterium to maintain the optimum fluidity of the membrane (e.g. following temperature change). </P>

Where are enzymes found in a bacterial cell