<P> Phosphate - buffered saline (abbreviated PBS) is a buffer solution commonly used in biological research . It is a water - based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate . The buffer helps to maintain a constant pH . The osmolarity and ion concentrations of the solutions match those of the human body (isotonic). </P> <P> PBS has many uses because it is isotonic and non-toxic to most cells . These uses include substance dilution and cell container rinsing . PBS with EDTA is also used to disengage attached and clumped cells . Divalent metals such as zinc, however, cannot be added as this will result in precipitation . For these types of applications, Good's buffers are recommended . </P> <P> There are many different ways to prepare PBS solutions (one of them is DPBS, or Dulbecco's phosphate - buffered saline, which has a lower phosphate concentration than standard PBS). Some formulations do not contain potassium and magnesium, while other ones contain calcium and / or magnesium . </P> <Table> The most common composition of PBS (1X) <Tr> <Th> Salt </Th> <Th> Concentration (mmol / L) </Th> <Th> Concentration (g / L) </Th> </Tr> <Tr> <Td> NaCl </Td> <Td> 137 </Td> <Td> 8.0 </Td> </Tr> <Tr> <Td> KCl </Td> <Td> 2.7 </Td> <Td> 0.2 </Td> </Tr> <Tr> <Td> Na HPO </Td> <Td> 10 </Td> <Td> 1.42 </Td> </Tr> <Tr> <Td> KH PO </Td> <Td> 1.8 </Td> <Td> 0.24 </Td> </Tr> </Table>

What is the difference between dpbs and pbs
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