<P> Chromosome conformation capture carbon copy (5C) detects interactions between all restriction fragments within a given region, with this region's size typically no greater than a megabase . This is done by ligating universal primers to all fragments . However, 5C has relatively low coverage . The 5C technique overcomes the junctional problems at the intramolecular ligation step and is useful for constructing complex interactions of specific loci of interest . This approach is unsuitable for conducting genome - wide complex interactions since that will require millions of 5C primers to be used . </P> <P> Hi - C uses high - throughput sequencing to find the nucleotide sequence of fragments . The original protocol used paired end sequencing, which retrieves a short sequence from each end of each ligated fragment . As such, for a given ligated fragment, the two sequences obtained should represent two different restriction fragments that were ligated together in the random ligation step . The pair of sequences are individually aligned to the genome, thus determining the fragments involved in that ligation event . Hence, all possible pairwise interactions between fragments are tested . </P> <P> Researchers attempt to study the extent of Hi - C's detection through a study focusing on screening primary brain tumours . Prior to screening tumours, Hi - C was primarily focused on cell lines . </P> <P> A number of methods use oligonucleotide capture to enrich Hi - C libraries for specific loci of interest . These methods include Capture - 3C, and Capture Hi - C . </P>

Hi-c a method to study the three-dimensional architecture of genomes